blog pai poenja

General SOP for Forensic Entomology -Preserving Entomology Evidences-

Posted in Forensic Entomology & DNA Barcoding by paibiopai on October 13, 2009
This is how we work on preserving forensic the entomology evidence..
just click here to see the rest..
Dead specimens à stored in 70% ethanol, all regardless whether they are immature or adult
Living specimens
Eggs killed and preserved in 70% ethanol
Larvae 1. stored in vial with tissue paper in it, and entry of air

2. then killed with hot water few minutes or in deep freezer apprx. -20oC for 1h

3. stored in ethanol 70%, DO NOT INSERT LIVING LARVAE DIRECTLY TO THE 70% ETHANOL

Pupae 1. stored in vial with tissue paper in it, and entry of air

2. then killed with hot water few minutes or in deep freezer apprx. -20oC for 1h

3. stored in ethanol 70%

Adults— 1. stored in vial with tissue paper in it, and an entry of air

2. then killed with hot water few minutes or in deep freezer apprx. -20oC for 1h

3. stored in ethanol 70%

(adults newly emerged from puparium should only be killed after the wings hardened and well-colored, apprx. 6 h)

Remnants document previous insect activities, stored in 70% ethanol

Dead specimens à stored in 70% ethanol, all regardless whether they are immature or adult

Living specimens à Eggs à killed and preserved in 70% ethanol

à Larvae à 1. stored in vial with tissue paper in it, and entry of air

2. then killed with hot water few minutes or in deep freezer apprx. -20oC for 1h

3. stored in ethanol 70%, DO NOT INSERT LIVING LARVAE DIRECTLY TO THE 70% ETHANOL

Pupae à 1. stored in vial with tissue paper in it, and entry of air

2. then killed with hot water few minutes or in deep freezer apprx. -20oC for 1h

3. stored in ethanol 70%

Adults à 1. stored in vial with tissue paper in it, and entry of air

2. then killed with hot water few minutes or in deep freezer apprx. -20oC for 1h

3. stored in ethanol 70%

(adults newly emerged from puparium should only be killed after the wings hardened and well-colored, apprx. 6 h)

Remnants à document previous insect activities, stored in 70% ethanol

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