blog pai poenja

Biological identifications through DNA barcodes

Posted in Forensic Entomology & DNA Barcoding by paibiopai on October 6, 2009

“Although much biological research depends upon species diagnoses, taxonomic expertise is collapsing. We are convinced that the sole prospect for a sustainable identification capability lies in the construction of systems that employ DNA sequences as taxon ‘barcodes’. We establish that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals. First, we demonstrate that COI profiles, derived from the low-density sampling of higher taxonomic categories, ordinarily assign newly analysed taxa to the appropriate phylum or order. Second, we demonstrate that species-level assignments can be obtained by creating comprehensive COI profiles. A model COI profile, based upon the analysis of a single individual from each of 200 closely allied species of lepidopterans, was 100% successful in correctly identifying subsequent specimens.When fully developed, a COI identification system will provide a reliable, cost-effective and accessible solution to the current problem of species
identification. Its assembly will also generate important new insights into the diversification of life and the rules of molecular evolution.” (Herbert et. al., 2003)

yoo, dna barcoding world has just begun.. all aplications regarding this thing are still unravelled.. now, it’s time for us to make a breakthrough,,, yeah..

you know, it’s my thesis topic,, dna barcode in forensic world.. I’m working this research in Eijkman Institute for Molecular Biology (although I’m a university student in Bandung Institute of Technology).. I’m lucky I can do this research in here with all complete equipments and tools that are needed here, and working under professional researcher in barcoding world.. I hope I can accomplish this research successfully on the end of January, and initiate the forensic dna barcoding research development in Indonesia..

wish me luck !! Bismillah !

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